Other

Part:BBa_K5107000:Design

Designed by: Georgios Retsinias   Group: iGEM24_DTU-Denmark   (2024-09-22)


HRE_minimal


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

As the system should be transcriptionally functional when the hormone is not present in the solution, we add the di-nucleotides GA between the T7 promoter and the HRE in order to keep high transcription level(extra Gs in the 3' end of the promoter)[1].


Source

The part is synthesised as member of the whole device we used in our cell free system obtaining as g-block from IDT.

References

1.Conrad, T., Plumbom, I., Alcobendas, M., Vidal, R., & Sauer, S. (2020). Maximizing transcription of nucleic acids with efficient T7 promoters. Communications Biology, 3(1). https://doi.org/10.1038/s42003-020-01167-x


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